Browsing by Author "Muniswaran, P.K.A."

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Coconut coir pith, available in abundance especially in tropical countries, was studied as a substrate for the production of cellulase[1,4(1,3;1,4)???D?glucan 4?glucanohydrolase, EC 3.2.1.4] and ??D?glucosidase(??D?glucoside glucohydrolase, EC 3.2.1.21) in solid state fermentation. The effects of fermentation time, nutrient level, substrate particle size and inoculum size have been examined for optimal production of these enzymes by the fungal strain Aspergillus niger NCIM 1005. The highest filter paper activity (FPA) of 4.11 IU g?1, carboxyl methyl cellulose (CMCase) activity of 15·55 IU g?1 and cellobiase activity of 9·31 IU g?1 were obtained after 7 to 8 days of fermentation. Reese and Mandel's mineral solution in the substrate to mineral solution ratio of 1:10 (w/v) supported high cellulase and cellobiase activities. An inoculum size of 20–50% (v/v) based on the volume of mineral medium and substrate average particle size of 375 ?m were optimum for enzyme production. Copyright © 1994 SCI
(Production of cellulases from coconut coir pith in solid state fermentation) Muniswaran, P.K.A.; Selvakumar, P.; Charyulu, N.C.L.N.
1994
Coconut pith, available in abundance, especially in tropical countries, can be an excellent new substrate for the production of cellulase enzyme by solid substrate cultivation of Trichoderma viride NCIM 1051. The effect of type of pretreatment, type and level of nutrient medium, inoculum volume, average substrate particle size, and time of fermentation on cellulase enzyme production by T. viride in coconut pith solid culture was studied. The hydrogen peroxide-pretreated coconut pith was found to be a better substrate. Reese and Mandels' mineral solution mixed with coconut pith in the ratio of 10:1 (v/w; ml g-1) supported maximum cellulase activity. The effect of inoculum volume on enzyme production was only marginal. An average substrate particle size of 375 ?m resulted in better enzyme production. The highest filter paper activity and carboxymethylcellulase activities of 4.27 and 12.05 IU g-1, respectively, were obtained in 7 days of fermentation, and the maximum cellobiase activity that could be obtained was 1.8 IU g-1 in 8 days. © 1994.
(Solid substrate fermentation of coconut coir pith for cellulase production) Muniswaran, P.K.A.; Charyulu, N.C.L.N.
1994
Production of cellulases from coconut coir pith in solid state fermentation
(1994) Muniswaran, P.K.A.; Selvakumar, P.; Charyulu, N.C.L.N.
Coconut coir pith, available in abundance especially in tropical countries, was studied as a substrate for the production of cellulase[1,4(1,3;1,4)???D?glucan 4?glucanohydrolase, EC 3.2.1.4] and ??D?glucosidase(??D?glucoside glucohydrolase, EC 3.2.1.21) in solid state fermentation. The effects of fermentation time, nutrient level, substrate particle size and inoculum size have been examined for optimal production of these enzymes by the fungal strain Aspergillus niger NCIM 1005. The highest filter paper activity (FPA) of 4.11 IU g?1, carboxyl methyl cellulose (CMCase) activity of 15 55 IU g?1 and cellobiase activity of 9 31 IU g?1 were obtained after 7 to 8 days of fermentation. Reese and Mandel's mineral solution in the substrate to mineral solution ratio of 1:10 (w/v) supported high cellulase and cellobiase activities. An inoculum size of 20 50% (v/v) based on the volume of mineral medium and substrate average particle size of 375 ?m were optimum for enzyme production. Copyright 1994 SCI
Solid substrate fermentation of coconut coir pith for cellulase production
(1994) Muniswaran, P.K.A.; Charyulu, N.C.L.N.
Coconut pith, available in abundance, especially in tropical countries, can be an excellent new substrate for the production of cellulase enzyme by solid substrate cultivation of Trichoderma viride NCIM 1051. The effect of type of pretreatment, type and level of nutrient medium, inoculum volume, average substrate particle size, and time of fermentation on cellulase enzyme production by T. viride in coconut pith solid culture was studied. The hydrogen peroxide-pretreated coconut pith was found to be a better substrate. Reese and Mandels' mineral solution mixed with coconut pith in the ratio of 10:1 (v/w; ml g-1) supported maximum cellulase activity. The effect of inoculum volume on enzyme production was only marginal. An average substrate particle size of 375 ?m resulted in better enzyme production. The highest filter paper activity and carboxymethylcellulase activities of 4.27 and 12.05 IU g-1, respectively, were obtained in 7 days of fermentation, and the maximum cellobiase activity that could be obtained was 1.8 IU g-1 in 8 days. 1994.