Browsing by Author "Manikandan, S."

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    Mechanistic understanding of biochar-bacteria system for enhanced chlorpyrifos bioremediation in water and soil medium
    (Elsevier B.V., 2024) Manikandan, S.; Mariyam, A.; Gowda, N.G.; Singh, A.; Nair, V.
    Chlorpyrifos (CP) is a commonly used chlorinated organophosphorus insecticide in agriculture, but its improper handling and long-term application have led to the contamination of groundwater, soil, and sediments. The use of functional microorganisms immobilized on biochar is an innovative and sustainable solution to this issue. In this study, CP-degrading Aeromonas veronii (A.veronii) was immobilized on rice husk biochar (ARHB) to investigate its efficacy in remediating CP-contaminated water and soil. Box-Behnken designs were employed to optimize the parameters, resulting in 96.25 % removal within 24 h in water medium. Adding 2 g kg−1 ARHB in soil achieved a maximum removal rate of 92.4 % within 42 days. Moreover, ARHB enhanced soil properties and plant growth through bio-augmentation and bio-stimulation. LC-MS analyses identified the degradation products, signifying a synergistic effect of bacterial metabolism and adsorption in the CP removal mechanism. These findings highlight the potential of immobilized A.veronii and biochar for efficient remediation of pesticide-contaminated environments. © 2024 Elsevier B.V.
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    Optimization and mechanistic study on bioremediation of Cr (VI) using microbial cell immobilized sugarcane bagasse biochar
    (Elsevier Ltd, 2024) Pallavi, P.; Manikandan, S.; Nair, V.
    Hexavalent chromium (Cr (VI)), a highly toxic heavy metal, contaminates water bodies due to its heavy usage in industrial processes. The present study proposes an environmentally friendly strategy for the bioremediation of Cr (VI) from water systems. Bacillus sp. was immobilized onto sugarcane bagasse biochar (BSCB) to remediate Cr (VI). The Cr (VI) removal process was optimized through one-factor-at-a-time (OFAT) and response surface methodology (RSM). Results showed that BSCB effectively removed 93.56 % of Cr (VI), with optimum conditions of 100 mg L−1 initial Cr (VI) concentration, 12 h incubation time, 40 °C temperature, pH 7.0, and 0.1 % BSCB dosage. The study also investigated reusability of BSCB across five consecutive cycles for the removal of Cr (VI) under optimized operating conditions, showcasing its consistent high performance compared to both sugarcane bagasse biochar (SCB) and free cells. BSCB maintained an average removal efficiency of 88.2 % across three cycles, outperforming SCB (56.67 %) and free cells (82.24 %). This immobilization approach enables better recovery of bacterial cells from water bodies and presents a sustainable, cost-effective bioremediation solution for Cr (VI) removal. © 2024 Elsevier Ltd