Browsing by Author "Arumugam, G.K.S."

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Differential delivery of vincristine to A549 cells using Chitosan capped silver nanoparticles under the pH trigger
(2017) Varadharajan, V.; Ramaswamy, A.; Bose, R.; Arumugam, G.K.S.
This study reports chitosan capped biogenically synthesized silver nanoparticles (Chit@AgNPs) of Diameter=12 nm for efficient delivery of vincristine (VCR) to A549 cells under the pH trigger. About 48% w/w of VCR could be loaded to Chit@AgNP without any conjugation system. The infrared spectroscopy indicated the presence of abundant quantities of the hydroxyl group on the surface of VCR loaded Chit@AgNP and it was confirmed by its negative zeta potential (-11.7 mV). Under in vitro condition, the release of VCR from Chit@AgNP into aqueous solution strictly followed first order kinetics with high preference to acidic pH. Significant cytotoxicity was observed in A549 cells than MDCK cells at p > 0-05 when incubated with VCR loaded Chit@AgNP, promoting high specificity towards cancer cells.
Differential delivery of vincristine to A549 cells using Chitosan capped silver nanoparticles under the pH trigger
(American Scientific Publishers order@aspbs.com, 2017) Venkatramanan, V.; Arulvel, A.; Bose, R.; Arumugam, G.K.S.
This study reports chitosan capped biogenically synthesized silver nanoparticles (Chit@AgNPs) of Diameter=12 nm for efficient delivery of vincristine (VCR) to A549 cells under the pH trigger. About 48% w/w of VCR could be loaded to Chit@AgNP without any conjugation system. The infrared spectroscopy indicated the presence of abundant quantities of the hydroxyl group on the surface of VCR loaded Chit@AgNP and it was confirmed by its negative zeta potential (-11.7 mV). Under in vitro condition, the release of VCR from Chit@AgNP into aqueous solution strictly followed first order kinetics with high preference to acidic pH. Significant cytotoxicity was observed in A549 cells than MDCK cells at p > 0-05 when incubated with VCR loaded Chit@AgNP, promoting high specificity towards cancer cells.
Extraction, optimization and characterization of collagen from sole fish skin
(2018) Arumugam, G.K.S.; Sharma, D.; Raj Mohan, Balakrishnan; Ettiyappan, J.B.P.
In this study, collagen was successfully extracted from marine waste i.e. Sole fish skin, which is available in the coastal area of Mangalore, India. The extraction process was optimized using One Variable at a Time (OVAT) and Response Surface Methodology (RSM) with Box-Behnken Design (BBD) was to achieve maximum yield and the extracted collagen was characterized. The optimal conditions to obtain highest collagen yield was determined to be, an acetic acid concentration of 0.54 M, NaCl concentration of 1.90 M, solvent/solid ratio of 8.97 ml/g and time of 32.32 h. The maximum collagen yield of 19.27 0.05 mg/g of fish skin was achieved under the optimal conditions. The analysis of variance and contour plots exhibited a significant interaction of all the selected variables over collagen extraction process. The SDS-PAGE (Sodium dodecyl sulfate - polyacrylamide gel electrophoresis) analysis suggested that the extracted collagen contained three ?-chains i.e. (?1)2, ?2 (M.W. 118, 116 kDa) and one ? chain (M.W. 200 kDa) which was similar to commercially available calfskin Type I collagen. FT-IR (Fourier Transform Infrared Spectroscopy) analysis confirmed the existence of helical arrangements of collagen. SEM (Scanning electron microscopy) observation revealed that the extracted collagen was in the form of fibrils with irregular linkages. 2018 Elsevier B.V.
Extraction, optimization and characterization of collagen from sole fish skin
(Elsevier B.V., 2018) Arumugam, G.K.S.; Sharma, D.; Mohan Balakrishnan, R.M.; JagadeeshBabu, P.E.
In this study, collagen was successfully extracted from marine waste i.e. Sole fish skin, which is available in the coastal area of Mangalore, India. The extraction process was optimized using One Variable at a Time (OVAT) and Response Surface Methodology (RSM) with Box-Behnken Design (BBD) was to achieve maximum yield and the extracted collagen was characterized. The optimal conditions to obtain highest collagen yield was determined to be, an acetic acid concentration of 0.54 M, NaCl concentration of 1.90 M, solvent/solid ratio of 8.97 ml/g and time of 32.32 h. The maximum collagen yield of 19.27 ± 0.05 mg/g of fish skin was achieved under the optimal conditions. The analysis of variance and contour plots exhibited a significant interaction of all the selected variables over collagen extraction process. The SDS-PAGE (Sodium dodecyl sulfate - polyacrylamide gel electrophoresis) analysis suggested that the extracted collagen contained three ?-chains i.e. (?1)2, ?2 (M.W. 118, 116 kDa) and one ? chain (M.W. 200 kDa) which was similar to commercially available calfskin Type I collagen. FT-IR (Fourier Transform Infrared Spectroscopy) analysis confirmed the existence of helical arrangements of collagen. SEM (Scanning electron microscopy) observation revealed that the extracted collagen was in the form of fibrils with irregular linkages. © 2018 Elsevier B.V.