Evaluation of feeding strategies for enhanced cell-associated tannase production by serratia ficaria dtc

Abstract

Batch studies on Cell-associated tannase production showed 2.6 U/L activity in the declining phase of growth in the bioreactor. It was observed that Cell-associated tannase production under declining phase was depending upon the bacterial biomass produced under exponential phase and gallic acid level. The peak production of enzyme was always accompanied by a sharp rise in dissolved oxygen concentration. Based on these observations, fed batch fermentation by feeding a mixture of nutrients (glucose and tryptose) and Dissolved oxygen (DO) based feeding strategy of gallic acid were designed. Nutrient feeding strategy showed 10 U/L of enzyme activity at 14th h of fermentation. DO based feeding strategy of gallic acid resulted in the production of 14.4 U/L enzyme activity in the 12th h of fermentation. The enzyme production rate of 1.2 U/L.h achieved in this mode was 4.6 fold greater than the values observed in batch process and 1.68 fold greater than the productivity achieved by feeding nutrients. Hence, DO based feeding strategy of gallic acid was proved to be an effective strategy for enhanced cell-associated tannase production by Serratia ficaria DTC. 2011 CAFET-INNOVA TECHNICAL SOCIETY. All rights reserved.